| Fatty Acid Content and Composition of Triacylglycerols of Chlorella kessleri
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Author:
Date:
2018-01-05
[Abstract] Triacylglycerols (TAGs) are esters formed from one glycerol and three fatty acids. TAGs are induced to accumulate in algal cells under environmental stress conditions including nutrient-limitation, hyperosmosis, and low temperature, for the storage of metabolic energy and carbon, and also for the consumption of excess energy (e.g., Hirai et al., 2016; Hayashi et al., 2017). Beside their physiological significance, the commercial utilization of algal TAG has been expected for the production of biodiesel, the methyl esters of fatty acids, from the aspect of carbon-neutral conception. The amounts of TAGs can be determined through quantitative measurement of their constituent fatty acids. This protocol consists of the following three parts: the first is the ...
[摘要] 三酰甘油（TAG）是由一种甘油和三种脂肪酸形成的酯。在包括营养限制，高渗和低温在内的环境胁迫条件下，TAG被诱导积累在藻细胞中，用于代谢能和碳的储存以及多余能量的消耗（例如， Hirai等人，2016; Hayashi等人，2017）。除了它们的生理意义之外，从碳中性概念的角度来看，藻类TAG的商业利用已经被期望用于生产生物柴油，即脂肪酸的甲酯。 TAG的量可以通过定量测定其构成脂肪酸来确定。该方案由以下三部分组成：首先是根据Bligh和Dyer（1959）的方法，使用有机溶剂，氯仿和甲醇从藻细胞中提取总脂质，其次是TAG的分离通过薄层色谱法（TLC）从其他脂质类别中分离出来，第三种是产生其组成型脂肪酸的甲基酯化衍生物，随后通过毛细管气相色谱法（GLC）对其进行定量。该协议改编自Sato和Tsuzuki（2011），用于绿藻的TAG分析， Chlorella kessleri 。
【背景】已经使用几种方法测定TAG的脂肪酸含量。简单而方便的方案包括用脂肪酶处理将TAG转化成甘油，随后通过酶产生与产生颜色或荧光的探针反应的产物测量甘油含量（McGowan等人，1983; ...
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| Seed Storage Reserve Analysis
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Author:
Date:
2014-10-20
[Abstract] One of the major goals of plant research is to improve crop yield, by for instance increasing seed oil or protein content. Besides this, extensive research is done to change seed fatty acid (FA) composition in order to make vegetable oils more suitable for specific purposes. To determine the effect of genetic changes on seed FA composition, oil, protein and sugar content it’s important to use standardised protocols to compare results between different research groups. Here we describe standardised methods for the analysis of seed FA composition, oil, protein and sugar content.
[摘要] 植物研究的主要目标之一是通过例如增加种子油或蛋白质含量来改善作物产量。 除此之外，还进行了广泛的研究以改变种子脂肪酸（FA）组成，以使植物油更适合于特定目的。 为了确定遗传变化对种子FA组成，油，蛋白质和糖含量的影响，使用标准化方案来比较不同研究组之间的结果是重要的。 在这里我们描述标准化的方法，用于分析种子FA组成，油，蛋白质和糖含量。
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