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ZebaTM Micro Spin Desalting Columns

Zeba TM微量离心脱盐柱

Company: Thermo Fisher Scientific
Catalog#: 89877
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Protein-ligand Binding Assay by Liquid Chromatography-Mass Spectrometry
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2011-08-20
[Abstract]  Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 da) to determine the affinity constant (kd) and the stoichiometry (bmax). da)="" to="" determine="" the="" affinity="" constant="" (kd)="" and="" the="" stoichiometry=""> [摘要]  在该方法中通过定量LC-MS测定蛋白质 - 小分子结合系数。 因此,传统的生化曲线用于建立已知蛋白质和小分子(<1000Da)之间的结合曲线,以确定亲和常数(Kd)和化学计量(Bmax)。

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