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Author:
Date:
2014-07-20
[Abstract] In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins et al., 2004; Chelysheva et al., 2012; Wang et al., 2009); furthermore, it is a crucial step for applying other cytogenetic methods including Fluorescence in situ hybridization (FISH) and immunostaining.
[摘要] 在这个协议，我们描述一种简单和有效的方法，在水稻花粉母细胞减数分裂染色体传播准备。 通过铺展本身的减数分裂染色体制备是植物细胞遗传学的重要技术（Higgins等人，2004; Chelysheva等人，2012; Wang等人，/em>，2009）; 此外，它是应用其他细胞遗传学方法包括荧光原位杂交（FISH）和免疫染色的关键步骤。
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