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Author:
Date:
2016-11-05
[Abstract] This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root extracts containing H2O2 can be mixed with a solution containing Amplex® Red reagent (10-acetyl-3,7-dihydrophenoxazine). In the presence of horseradish peroxidase, the Amplex® Red reagent reacts with H2O2 in a 1:1 stoichiometry. The resulting product is the red-fluorescent compound resorufin which can be detected fluorometrically or spectrophotometrically. Our protocol is based on ...
[摘要] 该方案描述了在拟南芥根组织中通过使用Amplex来测量过氧化氢（H 2 O 2 O 2）含量。 sup>红色过氧化氢/过氧化物酶测定试剂盒。当根组织被破坏并重新悬浮在磷酸盐缓冲液中时，H 2 O 2 O 2从细胞中释放。可以将获得的含有H 2 O 2 O 2的根提取物与含有Amplex Red试剂（10-乙酰基-3,7-二 - 。在辣根过氧化物酶的存在下，Amplex Red试剂与H 2 O 2以1:1的化学计量反应。所得产物是红色荧光化合物试卤灵，其可以通过荧光测定或分光光度法检测。我们的方案是基于Amplex ®红色过氧化氢/过氧化物酶测定试剂盒的手册，并描述了一步一步的程序，详细描述必要的控制和数据分析。我们还包括旨在帮助用户容易地用其自己的样品再现测定的方案，说明和实施例的修改。
[背景] 活性氧（ROS），例如H 2 O 2 O 2可以在该细胞是发育提示或应激条件的结果。在大量，ROS积累可能是有害的，通过造成细胞损伤。然而，增加的ROS产生也可以具有信号传导作用，并且作为控制下游细胞反应的第二信使。在植物中，对于许多非生物胁迫，例如干旱，盐度，温度胁迫和营养缺乏，已经显示ROS的信号传导作用（Mittler，2002; Mittler和Blumwald，2015; Xia等人）。 ，2015）。在我们最近的出版物Le ...
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Author:
Date:
2014-07-20
[Abstract] Heart failure occurring after acute myocardial infarction (MI) is among the main causes of death in western countries. Cell therapies, particularly those based on mesenchymal stem cells (MSC), represent one of the most promising approaches to repair damaged heart tissues. Several reports have provided evidences that injection of mesenchymal stem cells improved heart function following myocardial infarction (Shake et al., 2002; Zimmet and Hare, 2005; Zeng et al., 2007). Nevertheless, the mechanism(s) by which MSC exert their therapeutic action is far from being understood, and further knowledges in this field are required especially to optimize efficiency of current cardiac cell therapies. To assess the regenerative mechanisms developed by MSC in vitro, we ...
[摘要] 急性心肌梗死（MI）后发生的心力衰竭是西方国家死亡的主要原因。细胞疗法，特别是基于间充质干细胞（MSC）的那些，代表了修复受损心脏组织的最有希望的方法之一。几个报道提供了证据，注射间充质干细胞改善了心肌梗塞后的心脏功能（Shake等人，2002; Zimmet和Hare，2005; Zeng等人， 2007）。然而，MSC发挥其治疗作用的机制远未被理解，并且尤其需要在该领域中的进一步知识以优化当前心脏细胞治疗的效率。为了评估由MSC在体外形成的再生机制，我们开发了上述方法，其预期模拟梗塞心脏的典型微环境。该方法包括处于不良状态的小鼠终末分化心肌细胞与本文用作MSC模型的人多能脂肪来源干细胞（hMADS细胞）之间的物种错配共培养物。
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