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Hydrocortisone

氢化可的松

Company: Sigma-Aldrich
Catalog#: H0135
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Isolation of Mammary Epithelial Cells and Fibroblasts From Mouse Tumor
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2014-02-20
[Abstract]  Developing cancer therapeutics requires the ability to investigate their effects using in vitro models of a specific type of tumor. This protocol provides a method for the isolation and adoption to growth in culture of cells from primary tumors. This is particularly valuable for studying mouse models where original tumor cells can be evaluated, for example for gene modifications, and subsequently injected back to the same background mice to create more tumors for in vivo efficacy studies. [摘要]  开发癌症治疗剂需要使用特定类型肿瘤的体外模型研究它们的效果的能力。 该方案提供了用于分离和采用来自原发性肿瘤的细胞培养物中的生长的方法。 这对于研究可评估原始肿瘤细胞(例如基因修饰)并随后注射回相同背景小鼠以产生更多肿瘤用于体内效力研究的小鼠模型特别有价值。

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