{{'Search' | translate}}
 

CorningTM BioCoatTM Collagen I, Rat Tail Culture Dish

Corning TM BioCoat TM胶原I,大鼠尾培养皿

Company: Thermo Fisher Scientific
Catalog#: 08-774-8
Bio-protocol()
Company-protocol()
Other protocol()

Cell Surface Protein-protein Binding on COS-7 Cells
Author:
Date:
2014-01-05
[Abstract]  Examination of interactions between a transmembrane protein and a soluble protein by pull-down or immunoprecipitation assays can be tricky and complicated due to the detergent extraction of membrane proteins during the lysate preparation step. The choice and concentration of detergents must be determined empirically and the procedure can be burdensome. Here, we describe a simplified binding assay by expressing the membrane protein of interest in COS-7 cells and applying detergent-free solutions containing an extracellular protein to be tested. The binding is then examined by immunocytochemistry. [摘要]  通过下拉或免疫沉淀测定法检查跨膜蛋白和可溶性蛋白质之间的相互作用可能是棘手和复杂的,因为在裂解物制备步骤期间膜蛋白的去污剂提取。 洗涤剂的选择和浓度必须根据经验确定,并且该方法可能是繁重的。 在这里,我们描述简化的绑定测定通过表达感兴趣的COS蛋白的COS 7细胞和应用含有待测试的细胞外蛋白的无洗涤剂溶液。 然后通过免疫细胞化学检查结合。

Comments