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Kinetin

激动素

Company: Sigma-Aldrich
Catalog#: K0753
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MAMP-triggered Medium Alkalinization of Plant Cell Cultures
Author:
Date:
2020-04-20
[Abstract]  Plants recognize a wide variety of microbial molecules to detect and respond to potential invaders. Recognition of Microbe-Associated Molecular Patterns (MAMPs) by cell surface receptors initiate a cascade of biochemical responses that include, among others, ion fluxes across the plasma membrane. A consequence of such event is a decrease in the concentration of extracellular H+ ions, which can be experimentally detected in plant cell suspensions as a shift in the pH of the medium. Thus, similarly to reactive oxygen species (ROS) accumulation, phosphorylation of MAP kinases and induction of defense-related genes, MAMP-induced medium alkalinization can be used as a proxy for the activation of plant immune responses. Here, we describe a detailed protocol for the measurement of ... [摘要]  [摘要 ] 植物识别多种微生物分子以检测和响应潜在的入侵者,通过细胞表面受体识别微生物相关的分子模式(MAMP)引发一系列生化反应,其中包括跨血浆的离子通量此类事件的后果是细胞外H + 离子浓度降低,这可以通过实验检测到植物细胞悬浮液中培养基pH值的变化,因此类似于活性氧(ROS)积累, MAP激酶的磷酸化和防御相关基因的诱导,MAMP诱导的培养基碱化可以用作激活植物免疫反应的代理。在这里,我们描述了测量烟草BY-2细胞培养基碱化的详细方案两种不同MAMP处理后的悬浮液:衍生自真菌细胞壁的壳六聚体(NAG6; N-乙酰氨基葡萄糖)和鞭毛蛋白表位flg22 ,在细菌鞭毛发现。氏S分析提供了可靠和快速的平台来访问毫安时触发免疫(在MTI)在烟草悬浮细胞,并且可以很容易地适应其他植物物种以及其他毫安。

[背景 ] 在整个进化过程中,植物都具有检测微生物衍生分子并建立起能够抓住有害相互作用的免疫反应的能力(Boutrot和Zipfel,2017年)。诱导此类免疫反应的微生物相关分子模式(MAMP)通常被广泛保存。微生物的结构成分,例如真菌的几丁质和细菌的鞭毛蛋白(Cook 等人,2015)。胞外植物受体对MAMP的识别会导致MAMP触发的免疫(MTI),从而阻止微生物入侵并维持植物健康。(Böhmet al。,2014)。

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Shoot Regenerative Capacity Assays in Arabidopsis and Tobacco
Author:
Date:
2016-03-05
[Abstract]  Plant regeneration refers to a process through which an explant is differentiated to a whole plant under certain conditions. It has been shown that two plant hormones, auxin and cytokinin, play critical roles within this process (Duclercq et al., 2011). Cytokinin induces shoot regeneration, whereas auxin promotes root production. In addition to hormones, recent study has revealed that age cue serves as a common element behind plant cell totipotency (Toledano et al., 2012). Here we present an easy protocol to assess the shoot regenerative capacity of Arabidopsis and tobacco leaves of different ages. [摘要]  植物再生是指在某些条件下外植体分化成整株植物的过程。 已经表明,两种植物激素,植物生长素和细胞分裂素在该过程中起关键作用(Duclercq等人,2011)。 细胞分裂素诱导芽再生,而生长素促进根生产。 除了激素,最近的研究已经揭示年龄提示作为植物细胞全能性背后的共同元素(Toledano等人,2012)。 在这里我们提出了一个简单的协议,以评估不同年龄的拟南芥和烟草叶的射线再生能力。

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