|
Author:
Date:
2014-01-05
[Abstract] This is a protocol to produce stable transgenic potato plants (Solanum tuberosum cv. Désirée) by Agrobacterium-mediated genetic transformation, which is established based on a method described by (Jung et al., 2005) with some modifications. Agrobacterium tumefaciens strain LBA4404 carrying the desired construct is used to infect internodal explants to produce stable transgenic potato plants. Plantlet screening and molecular analyses are employed to confirm the expression of transgene in generated transgenic potato lines.
[摘要] 这是通过土壤杆菌介导的遗传转化产生稳定的转基因马铃薯植物（马铃薯（Solanum tuberosum）cv.Désirée）的方案，其基于由（Jung& em。等人，2005），其具有一些修改。 携带所需构建体的根癌农杆菌菌株LBA4404用于感染节间外植体以产生稳定的转基因马铃薯植物。 植物筛选和分子分析用于确认转基因在所产生的转基因马铃薯品系中的表达
|