{{'Search' | translate}}
 

EpiTect Bisulfite Kit

EpiTect亚硫酸氢盐试剂盒

Company: QIAGEN
Catalog#: 59104
Bio-protocol()
Company-protocol()
Other protocol()

Library Construction for Genome-wide Bisulfite Sequencing in Plants
Author:
Date:
2013-12-20
[Abstract]  DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. This protocol describes how to prepare libraries of genomic DNA for whole-genome bisulfite sequencing in Arabidopsis, which could be adapted for use in other plant species. [摘要]  DNA甲基化是研究最多的表观遗传修饰,其涉及将甲基添加到DNA中胞嘧啶残基的碳-5位置。 DNA甲基化对于基因表达的调节是重要的。 亚硫酸氢盐测序是确定真核生物全基因组DNA甲基化谱的金标准技术。 该协议描述了如何在拟南芥中制备用于全基因组亚硫酸氢盐测序的基因组DNA文库,其可以适用于其他植物物种。

Comments