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MICRO CAPILLARY PIPETTES

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Company: Kimble Chase Life Science
Catalog#: 71900-10
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Paper Chromatography as Exemplified by Separation of Urocanic Acid and Deaminohistidine
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2013-06-20
[Abstract]  Paper chromatography is an ancient technique to separate low molecular mass compounds based on their distribution between mobile phase (solvent) and stationary phase (cellulose and cellulose-bound water). Paper chromatography has been largely replaced by thin layer chromatography and high performance liquid chromatography as the latter methods have higher resolution capability. Nevertheless due to low cost and availability of great number of protocols for separation of various compounds, paper chromatography is still a powerful analytical tool. In the current protocol this technique is exemplified by separation of urocanic acid and deaminohistidine. [摘要]  纸色谱是一种基于其在流动相(溶剂)和固定相(纤维素和纤维素结合水)之间的分布而分离低分子量化合物的古老技术。 纸色谱已经在很大程度上被薄层色谱和高效液相色谱取代,因为后者的方法具有更高的分离能力。 然而,由于低成本和大量用于分离各种化合物的方案的可用性,纸色谱法仍然是一种强大的分析工具。 在目前的方案中,该技术通过尿刊酸和脱氨基组氨酸的分离来举例说明。

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