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Alginic acid sodium salt from brown algae

来自褐藻的藻酸钠盐

Company: Sigma-Aldrich
Catalog#: A2158
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Viral Immunofluorescence with Rift Valley Fever Virus Infected MEFs in a 96 Well Plate
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2012-12-05
[Abstract]  Immunofluorescence is a method to detect viral infection in multiple types of host cells. This procedure can be adapted for both high-throughput and low-throughput assays for any virus for which there are antibodies available. Time of infection and virus multiplicity of infection (MOI) vary and should be optimized for each virus and host cell type. Here we give an example of viral immunofluorescence in a 96 well plate with a Rift Valley fever virus (RVFV, strain MP12) infection in mouse embryonic fibroblasts (MEF). [摘要]  免疫荧光是检测多种类型的宿主细胞中的病毒感染的方法。 该方法可适用于任何具有可用抗体的病毒的高通量和低通量测定。 感染时间和病毒感染复数(MOI)不同,应该对每种病毒和宿主细胞类型进行优化。 在这里我们给出在小鼠胚胎成纤维细胞(MEF)中使用Rift Valley热病毒(RVFV,菌株MP12)感染的96孔板中的病毒免疫荧光的实例。

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