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Imidazole

咪唑

Company: Sigma-Aldrich
Catalog#: 436151
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Purification of His-ubiquitin Proteins from Mammalian Cells
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Date:
2012-09-05
[Abstract]  This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just the p53-ubiquitinate. The present protocol can be used in general for His-tag proteins expressed in mammalian cells. [摘要]  该方案用于纯化His标签泛素缀合的蛋白。 在这种特殊情况下,细胞用His标签泛素和p53转染,这允许我们使用His标签进行纯化,并使用针对p53的抗体显示WB以仅观察p53泛素化。 本方案通常可用于在哺乳动物细胞中表达的His标签蛋白。

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