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Buffer QC

缓冲液QC

Company: QIAGEN
Catalog#: 19055
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Yeast DNA Replication 2D Gel Protocol
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Date:
2012-07-05
[Abstract]  Two-dimensional agarose gel electrophoresis (2D gel) analysis is used extensively as a method to detect origins of replication. Here, I present a simplified method for the isolation of yeast genomic DNA for 2D gel analysis from a small number of yeast cells. This DNA isolation method is simpler and less time consuming than the traditional method that involves CsCl density gradient centrifugation. This method could be modified for 2D gel analysis in other organisms as well. [摘要]  二维琼脂糖凝胶电泳(2D凝胶)分析广泛用作检测复制起点的方法。 在这里,我提出了一个简单的方法分离酵母基因组DNA的2D凝胶分析从少量的酵母细胞。 这种DNA分离方法比涉及CsCl密度梯度离心的传统方法更简单和更省时。 这种方法可以修改为其他生物体的2D凝胶分析。

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