| Characterization of HBV Isolates from Patient Serum Samples and Cloning
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Author:
Date:
2015-12-20
[Abstract] Hepatitis B virus (HBV) mutants can lead to vaccine failure, diagnostic failure of HBV detection, increase viral replication and resistance to antiviral agents. To study the biological characteristics of these mutations may contribute to our knowledge on viral pathogenesis. Therefore, it is essential to isolate and characterize HBV strains from patients. Here we describe the experimental methods to isolate and clone HBV DNA from patient serum. The method will facilitate isolation and functional analysis of new HBV variants.
[摘要] 乙型肝炎病毒（HBV）突变体可导致疫苗失败，HBV检测的诊断失败，增加病毒复制和对抗病毒剂的抗性。 研究这些突变的生物学特性可能有助于我们对病毒发病机制的了解。 因此，必须从患者中分离和表征HBV病毒株。 在这里我们描述从患者血清中分离和克隆HBV DNA的实验方法。 该方法将促进新的HBV变体的分离和功能分析。
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| In vitro Drug Susceptibility Assay for HBV Using Southern Blotting
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Author:
Date:
2015-04-20
[Abstract] Antiviral agents for the suppression of hepatitis B virus (HBV) have been used for treating chronic hepatitis B. However, the emergence of drug-resistant HBV is still a major problem for antiviral treatment. To identify and characterize the drug-resistant HBV, the construction of HBV replicon and in vitro drug susceptibility assay are essential. Here we describe the experimental methods to study drug-resistant HBV.
[摘要] 用于抑制乙型肝炎病毒（HBV）的抗病毒剂已经用于治疗慢性乙型肝炎。然而，耐药性HBV的出现仍然是抗病毒治疗的主要问题。 为了鉴定和表征耐药性HBV，构建HBV复制子和体外药物敏感性测定是必需的。 在这里我们描述研究耐药性HBV的实验方法。
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| A Simple RNA Preparation Procedure from Yeast for Northern Blot Using Hot Phenol
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Author:
Date:
2012-06-20
[Abstract] Compared to several expensive RNA extraction kits, the following protocol provides an economic and simple method for researchers to extract yeast RNA. This method can achieve RNA quality that is sufficient for most northern blot studies in yeast.
[摘要] 与几种昂贵的RNA提取试剂盒相比，以下方案为研究人员提取酵母RNA提供了经济和简单的方法。 这种方法可以实现RNA质量，足以用于酵母中的大多数Northern印迹研究。
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