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Company: Thermo Fisher Scientific
Catalog#: A995-4
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Establishment of a Fusarium graminearum Infection Model in Arabidopsis thaliana Leaves and Floral Tissues
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2016-07-20
[Abstract]  Fusarium graminearum (Fg) is the causal agent of Fusarium head blight disease of wheat (Triticum aestivum), oats (Avena sativa) and barley (Hordeum vulgare), which targets the floral tissues and thereby adversely impacts grain yield and quality. Mycotoxins produced by F. graminearum further limit the consumability of infected grain. In the laboratory, F. graminearum also has the ability to colonize both leaves and inflorescence tissues of Arabidopsis thaliana. The interaction between A. thaliana and F. graminearum makes available a large array of genetic and molecular tools to study the interaction between plants and F. graminearum to elucidate plant genes and pathways that contribute to resistance, ... [摘要]  禾谷镰刀菌(Fg)是小麦(小麦),燕麦( Avena sativa )和大麦镰刀菌 ),其针对花组织,从而不利地影响谷物产量和质量。由F生产的霉菌毒素。禾谷镰菌进一步限制了感染谷物的消耗性。在实验室,F。禾谷镰刀菌也具有定居拟南芥的叶和花序组织的能力。 A之间的交互。 thaliana 和 F。禾谷镰刀菌(graminearum)提供了大量遗传和分子工具来研究植物和真菌之间的相互作用。禾本科(Graminearum)来阐明植物基因和促进抗性的途径,以及研究真菌如何靶向植物基因和促进疾病的机制。下面描述的方法允许有效地感染拟南芥叶和花序,以及评价疾病进展和真菌生长。拟南芥中的病害传播可以通过叶组织的萎黄病和花序组织的包括真菌团块在花序组织表面上的病害表型的视觉观察来容易地监测。可以通过聚合酶链反应(PCR)和定量实时PCR(qPCR)测量宿主组织中的Fg DNA的相对量来进一步监测真菌生长。

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