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Calcium Chloride

氯化钙
Company: AppliChem
Catalog#: A3652
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Isolation and Primary Culture of Adult Mouse Cardiac Fibroblasts
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2016-07-05
[Abstract]  Fibroblasts are often used as a feeder layer for progenitor or stem cells in co-culture systems. In the heart fibroblasts are important for cardiac development, homeostasis, and remodelling. They provide cardiomyocytes and progenitor cells not only with nutrition but also secrete extracellular matrix that forms the microenvironment that ensures cell survival and function. Although different kinds of mouse fibroblasts have been used in co-cultures (embryonic, skin and cardiac fibroblasts) adult mouse cardiac fibroblasts (AMCFs) create the closest microenvironment to the adult murine heart for culturing adult mouse cardiac progenitor cells. This protocol describes the isolation of cardiac fibroblasts from adult mouse hearts as well as their maintenance in culture. [摘要]  成纤维细胞通常用作共培养系统中的祖细胞或干细胞的饲养层。 在心脏成纤维细胞对于心脏发育，体内平衡和重塑是重要的。 他们提供心肌细胞和祖细胞不仅与营养，而且分泌细胞外基质，形成微环境，确保细胞的生存和功能。 尽管不同种类的小鼠成纤维细胞已用于共培养（胚胎，皮肤和心脏成纤维细胞），但是成年小鼠心脏成纤维细胞（AMCFs）为培养成年小鼠心脏祖细胞产生了与成年鼠心脏最接近的微环境。 该协议描述了从成年小鼠心脏分离心脏成纤维细胞以及它们在培养物中的维持。

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