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Tris/HCl

Tris盐酸盐

Company: Sigma-Aldrich
Catalog#: 10812846001
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MNase Digestion for Nucleosome Mapping in Neurospora
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Date:
2016-06-05
[Abstract]  Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin without a requirement of a nuclear fractionation step. [摘要]  通过微球菌核酸酶MNase消化染色质,然后高通量测序允许我们确定核小体在基因组上的位置和占据。 在这个协议中,我们描述了MNase消化丝状真菌粗糙链孢霉染色体的优化条件,而不需要核分馏步骤。

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