KCl (2 M)

KCl（2M）

Company: Thermo Fisher Scientific

Catalog#: AM9640G

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MNase Digestion for Nucleosome Mapping in Neurospora

Author:

Cigdem Sancar, Gencer Sancar and Michael Brunner,

Date:

2016-06-05

[Abstract] Digestion of chromatin by micrococcal nuclease MNase followed by high throughput sequencing allows us to determine the location and occupancy of nucleosomes on the genome. Here in this protocol we have described optimized conditions of MNase digestion of filamentous fungus Neurospora crassa chromatin without a requirement of a nuclear fractionation step. [摘要] 通过微球菌核酸酶MNase消化染色质，然后高通量测序允许我们确定核小体在基因组上的位置和占据。在这个协议中，我们描述了MNase消化丝状真菌粗糙链孢霉染色体的优化条件，而不需要核分馏步骤。

A Surface Plasmon Resonance Method to Study HCV NS5B Inhibitors

Author:

Melanie Wong and Giuseppe A. Papalia,

Date:

2014-02-20

[Abstract] Surface Plasmon Resonance (SPR) technology is a well-established platform used to evaluate the kinetic parameters of protein-small molecule interactions. Below, we describe the use of the ProteOn XPR36 biosensor from Bio-Rad (Hercules, CA) to evaluate the binding of small molecule inhibitors to recombinant NS5B protein. The high pI (> 9) of this construct allows for chemical immobilization using HEPES-buffered saline at pH 7.5. This is in contrast to traditional biosensor protocols that use both low pH and ionic strength. The use of a more physiological buffer to immobilize this enzyme leads to improved surface activity. [摘要] 表面等离子体共振（SPR）技术是一个成熟的平台，用于评估蛋白质 - 小分子相互作用的动力学参数。下面，我们描述使用来自Bio-Rad（Hercules，CA）的ProteOn XPR36生物传感器来评价小分子抑制剂与重组NS5B蛋白的结合。该构建体的高pI（> 9）允许使用pH7.5的HEPES缓冲盐水进行化学固定。这与使用低pH和离子强度的传统生物传感器方案相反。使用更多生理缓冲液来固定该酶导致改善的表面活性。

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KCl (2 M)

KCl（2M）

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