ViiA 7 Real-Time PCR System with 384-Well Block

ViiA 7实时PCR系统，384孔板

Company: Thermo Fisher Scientific

Catalog#: 4453536

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Respiratory Syncytial Virus Infection in Mice and Detection of Viral Genomes in the Lung Using RT-qPCR

Author:

Yan Sun and Carolina B. López,

Date:

2016-05-20

[Abstract] Respiratory syncytial virus (RSV) is a single-stranded negative sense RNA virus that belongs to the paramyxovirus family. RSV infections lead to a variety of clinical outcomes ranging from a mild “cold-like disease” to death. Infection is usually more severe in infants and the elderly. RSV is associated with the development and exacerbation of chronic lung conditions including asthma, and it is a major cause of hospitalizations in infants. Because of its clinical relevance, experimental animal models to study RSV in vivo are needed. The most common and accessible animal model in research laboratories is the mouse. However, commonly use RSV strains poorly establish infection in mice and thus titration of the virus from mouse lungs to confirm infection is not sensitive ... [摘要] 呼吸道合胞病毒（RSV）是属于副粘病毒家族的单链负义RNA病毒。 RSV感染导致各种临床结果，从轻度"冷样疾病"到死亡。婴儿和老年人的感染通常更严重。 RSV与慢性肺病（包括哮喘）的发展和加重有关，并且是婴儿住院的主要原因。由于其临床相关性，需要用于在体内研究RSV的实验动物模型。研究实验室中最常见和可获得的动物模型是小鼠。然而，通常使用RSV毒株在小鼠中很少建立感染，因此从小鼠肺中滴定病毒以确认感染不足以检测早期病毒感染。在这里我们详细讨论如何感染BALB/c小鼠与RSV和如何使用逆转录定量PCR（RT-qPCR）肺中检测RSV基因组。这种方法允许早在感染后1天检测病毒基因组（图2所示），而传统的TCID 50不能检测到显着的病毒，直到感染后2天。值得注意的是，尽管灵敏度较高，但基因组RT-qPCR仅显示病毒基因组的产生，因此该测定的阳性结果不是感染性病毒颗粒产生的证据。

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ViiA 7 Real-Time PCR System with 384-Well Block

ViiA 7实时PCR系统，384孔板

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