|
Author:
Date:
2016-04-20
[Abstract] Cyclic AMP (cAMP) is a ubiquitous secondary signaling molecule, commonly associated with many bacterial processes, including the regulation of virulence factors, such as biofilms, pellicles and motility (Wolfgang, 2003). The quantity of available cAMP is controlled by the interplay between the synthesis of adenosine triphosphate (ATP) to cAMP by adenylyl cyclases, and the degradation of cAMP by phosphodiesterase (McDonough et al., 2012). Adequate quantification of cAMP levels within a bacterial cell is an important step in identifying the impact that secondary signaling molecules play on the regulatory pathway within the cell. The principle of this method is to measure total cAMP levels within a bacterial cell, using crude bacterial whole cell lysate. The Cyclic AMP XPTM ...
[摘要] 环AMP（cAMP）是普遍存在的次级信号分子，通常与许多细菌过程相关，包括调节毒力因子，如生物膜，薄膜和运动性（Wolfgang，2003）。可用的cAMP的量由腺苷酸环化酶对cAMP的腺苷三磷酸（ATP）合成和磷酸二酯酶对cAMP的降解之间的相互作用控制（McDonough等人，2012）。细菌细胞内cAMP水平的充分量化是鉴定次级信号传导分子对细胞内调节途径起作用的重要步骤。该方法的原理是使用粗细菌全细胞裂解物测量细菌细胞内的总cAMP水平。本方案中使用的环状AMP XP sup试剂盒最初设计用于测定真核细胞中cAMP的水平，然而，用于涂覆孔的抗体将与来自任何物种的cAMP反应并因此可用于测定细菌细胞中的水平。这里描述的原核细胞中cAMP的测量是产生可定量结果的简单且成本有效的方法。
|