| Antifungal and Zearalenone Inhibitory Activity of Ocimum sanctum L. Essential Oil on Fusarium graminearum Determined by UHPLC and RT-qPCR
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Author:
Date:
2016-08-05
[Abstract] Fusarium graminearum has been given special attention in the context of agricultural commodities due to its ability to grow in diverse climatic conditions, and to produce different mycotoxins including zearalenone (ZEA) and type-B trichothecenes, which cause ill health effects on humans, animals and plants. The application of synthetic antifungal agents for the control of F. graminearum result in negative health impacts in livestock and humans and the upsurge of resistant organisms as well. Therefore, there is a need to propose proper food grain management practices, including the application of herbal antifungal and mycotoxin controlling agents, to reduce the growth of toxigenic F. graminearum as well as the production of ZEA in agricultural commodities. Ocimum ...
[摘要] 由于其在多种气候条件下生长的能力，并且产生不同的真菌毒素，包括玉米赤霉烯酮（ZEA）和B型单端孢霉烯，因此在农业商品的背景下已经特别关注禾谷镰刀菌对人类，动物和植物的健康影响。合成抗真菌剂用于控制F的应用。禾谷镰菌对家畜和人类的健康造成负面影响，以及抗性生物体的高涨。因此，需要提出适当的食物谷物管理实践，包括应用草本抗真菌剂和真菌毒素控制剂以减少产毒的F的生长。禾谷镰菌以及在农产品中生产ZEA。 罗勒属圣地也被称为圣罗勒或图尔西被广泛用作阿育吠陀的药用植物。目前的协议表明量化em的抗真菌活性。圣地牙本香精油（OSEO），如由降低的F所反映的。禾谷镰刀菌生长和ZEA生产。 OSEO的抗真菌活性通过微孔稀释法进行，并通过扫描电子显微镜方法定量进一步验证。通过定量逆转录PCR（RT-qPCR）和超高效液相色谱（UHPLC）方法从F的肉汤培养物分析OSEO对ZEA产生的影响。禾本科。 OSEO的抗真菌毒性功效直接在F上评估。禾谷镰菌接种的玉米谷物。该方案有效地评估OSEO作为抗真菌侵袭和ZE生产的草药拮抗剂的活性。禾本科。该方案可用于测试多种草药化合物对抗F的抗真菌活性。禾谷镰刀菌或对其他霉菌毒素真菌进行修饰，这是对食品安全和加工工业的重要干预，其中真菌侵染是主要关注的问题。
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| Measurement of Intracellular cAMP Levels Using the Cyclic Nucleotide XP Enzymatic Immunoassay Kit in Bacteria
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Author:
Date:
2016-04-20
[Abstract] Cyclic AMP (cAMP) is a ubiquitous secondary signaling molecule, commonly associated with many bacterial processes, including the regulation of virulence factors, such as biofilms, pellicles and motility (Wolfgang, 2003). The quantity of available cAMP is controlled by the interplay between the synthesis of adenosine triphosphate (ATP) to cAMP by adenylyl cyclases, and the degradation of cAMP by phosphodiesterase (McDonough et al., 2012). Adequate quantification of cAMP levels within a bacterial cell is an important step in identifying the impact that secondary signaling molecules play on the regulatory pathway within the cell. The principle of this method is to measure total cAMP levels within a bacterial cell, using crude bacterial whole cell lysate. The Cyclic AMP XPTM ...
[摘要] 环AMP（cAMP）是普遍存在的次级信号分子，通常与许多细菌过程相关，包括调节毒力因子，如生物膜，薄膜和运动性（Wolfgang，2003）。可用的cAMP的量由腺苷酸环化酶对cAMP的腺苷三磷酸（ATP）合成和磷酸二酯酶对cAMP的降解之间的相互作用控制（McDonough等人，2012）。细菌细胞内cAMP水平的充分量化是鉴定次级信号传导分子对细胞内调节途径起作用的重要步骤。该方法的原理是使用粗细菌全细胞裂解物测量细菌细胞内的总cAMP水平。本方案中使用的环状AMP XP sup试剂盒最初设计用于测定真核细胞中cAMP的水平，然而，用于涂覆孔的抗体将与来自任何物种的cAMP反应并因此可用于测定细菌细胞中的水平。这里描述的原核细胞中cAMP的测量是产生可定量结果的简单且成本有效的方法。
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