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Author:
Date:
2016-01-20
[Abstract] Calcium plays important roles in maintaining plant cellular structure and also acts as a key secondary messenger in intercellular signaling. Thirty years ago, methods of detecting calcium in sub-cellular level had been established (Stockwell and Hanchey, 1982; Borgers et al., 1982) and reviewed extensively (Wick and Heplerm, 1982). We had used the method of testing calcium localization in salt tolerance improved transgenic alfalfa plant (Zhang and Wang, 2015). Here, we describe the protocol of testing calcium deposition by staining with potassium pyroantimonate (PPA) in detail, which was adapted from former reports (Stockwell and Hanchey, 1982; Borgers et al., 1982). The principle of this protocol is that the Ca2+ can react with antimonite and from black ...
[摘要] 钙在维持植物细胞结构中起重要作用，并且还在细胞间信号传导中作为关键的第二信使。三十年前，已经建立了在亚细胞水平检测钙的方法（Stockwell和Hanchey，1982; Borgers等人，1982）并广泛地综述（Wick和Heplerm，1982）。我们使用测试钙定位的方法在耐盐改良的转基因苜蓿植物中（Zhang和Wang，2015）。在这里，我们描述了通过用详细的焦锑酸钾（PPA）染色测试钙沉积的方案，其改编自以前的报道（Stockwell和Hanchey，1982; Borgers等人，1982）。该方案的原理是Ca 2+ 2+可以与锑矿和黑色颗粒反应，这可以在透射电子显微镜下观察到。该协议包括植物组织的常见显微操作技术，用透射电子显微镜和照相观察。
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