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Polystyrene Microplates

聚苯乙烯微孔板

Company: Thermo Fisher Scientific
Catalog#: 08-772-1
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Dye Labeling of Live Nippostrongylus brasiliensis Larvae for Visualization in Host Tissue
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2016-02-20
[Abstract]  Visualization of the interaction between parasitic nematodes and their host enables a better understanding of the development of the nematode during the infectious stages of its life cycle and of the effects of host response on nematode integrity in tissues. Appropriate live imaging of these nematode/host interactions, to date has been hindered by the lack of appropriate molecular tools or efficient labeling agents. Here, we present techniques for the live labeling of the nematode parasite Nippostrongylus brasiliensis (N. brasiliensis) that allows visualization of the parasite in the mouse host for up to 24 h. The external sheath can be labeled with CFSE allowing infective larvae to be identified and followed until the stage of exsheathment. The internal labeling of ... [摘要]  寄生线虫与其宿主之间的相互作用的可视化使得能够更好地理解线虫在其生命周期的感染阶段期间的发展以及宿主对组织中线虫完整性的反应的影响。 这些线虫/宿主相互作用的适当的活体成像迄今为止由于缺乏合适的分子工具或有效的标记试剂而受到阻碍。 在这里,我们提出了线虫寄生虫(Nippostrongylus brasiliensis)( N。brasiliensis )的活标签的技术,其允许在小鼠宿主中可视化寄生虫长达24小时。 外鞘可以用CFSE标记,允许感染性幼虫被鉴定和跟踪,直到呕吐的阶段。 感染性寄生虫的内部标记可以通过摄取NY微球来进行。 蠕虫可以继续被识别长达24小时以下的exshement。 这应适用于其他寄生线虫。

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