| In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
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Author:
Date:
2016-02-20
[Abstract] Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood vessels (leukocyte mimicry). The shear resistant adhesion is mediated by high affinity interactions between endothelial adhesion molecules and their counter receptor ligand expressed on circulating cells. Thus, weak interaction results in a rapid detachment of circulating cells from endothelium. Despite the critical role of vascular adhesion of cancer cells in hematogenous metastasis, our knowledge regarding this process has been limited due to ...
[摘要] 血液转移是转移性癌症死亡的主要原因。循环肿瘤细胞对血流内的血管内皮细胞表面的剪切粘附是细胞外渗和进一步组织侵入的必要步骤。这类似于白细胞用于粘附到发炎血管的方法（白细胞模拟）。剪切抗性粘附由内皮粘附分子和其在循环细胞上表达的其受体配体之间的高亲和力相互作用介导。因此，弱相互作用导致循环细胞从内皮快速分离。尽管癌细胞在血源性转移中的血管粘附的关键作用，我们关于该过程的知识由于难以在体外模拟动态流动条件而受到限制。为了更好地了解癌细胞对内皮的剪切抗性粘附，我们开发了一种用于测量在生理流动条件下循环肿瘤细胞对内皮细胞的剪切抗性粘附的方案，通过适应良好确立的流动粘附测定炎症细胞。该技术可用于评价1）癌细胞的抗剪切粘附能力和2）支持癌细胞粘附所必需的内皮粘附分子（Kang等人，2015）。
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| Pancreatic Acinar Cell 3-Dimensional Culture
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Author:
Date:
2013-10-05
[Abstract] Normal pancreatic acinar cells are difficult to maintain on traditional plastic culture surfaces due to their physical properties of housing large quantities of digestive enzymes and the formation of intercellular tight junctions and gap junctions (Apte and Wilson 2005; Rukstalis et al., 2003). However, placing primary acinar cells within a 3-dimensional matrix (3D-culture) maintains the cells for sufficient time so that they can be monitored for physiological changes to different stimuli. We have used a modified collagen 3D-culture system that has been adapted from Means et al. (2005) to model the very early events associated with pancreatic cancer development. In this model, KrasG12D-expressing pancreatic acinar cells, or wildtype acinar cells treated with ...
[摘要] 正常的胰腺腺泡细胞难以保持在传统的塑料培养表面上，因为其具有容纳大量消化酶和形成细胞间紧密连接和间隙连接的物理性质（Apte和Wilson 2005; Rukstalis等人， ，2003）。然而，将原代腺泡细胞放置在三维基质（3D培养）中保持细胞足够的时间，使得它们可以被监测对不同刺激的生理变化。我们已经使用改良的胶原三维培养系统，其已经从Means等人（2005）改编以模拟与胰腺癌发展相关的非常早期的事件。在该模型中，表达Kras G12D 的表达胰腺腺泡细胞或用EGFR依赖性生长因子（即，TGFα）处理的野生型腺泡细胞转化成导管囊肿，在胰腺上皮内瘤形成（PanIN）和胰腺导管腺癌（PDAC）形成之前的腺泡至导管化生（ADM）阶段（Means等人，2005; Shi等人，/em>，2013）。
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