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Ammonium Sulfate AR

硫酸铵AR

Company: Chem Supply
Catalog#: AA014-500G
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Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
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2016-01-20
[Abstract]  Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at qualitative level and quantitative level. The protocol includes separating proteins isolated from developing endosperm with native-PAGE containing glycogen from oyster, incubating protein gels with ADP-glucose solution, and staining gels with iodine solution. The method allows researchers to compare the levels or changes of starch synthase activities. [摘要]  淀粉合酶是涉及谷物淀粉合成的一类关键酶,其将葡萄糖从ADP-葡萄糖转移到预先存在的支链淀粉的α-(1-4) - 葡萄糖基链的非还原端。 这个协议是高度可重复的测定淀粉合成酶I和IIIa在小麦和大麦胚乳的定性水平和定量水平的活动。 该方案包括从发育的胚乳分离的蛋白质与来自牡蛎的含有糖原的天然PAGE,用ADP-葡萄糖溶液孵育蛋白质凝胶,并用碘溶液染色凝胶。 该方法允许研究人员比较淀粉合酶活性的水平或变化。

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