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Newclassic MS-TS Analytical Balance

新经典MS-TS分析天平

Company: Cole-Parmer
Catalog#: MS204TS
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Measurement of Uptake and Root-to-Shoot Distribution of Sulfate in Arabidopsis Seedlings
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2016-01-05
[Abstract]  Sulfur is an essential macronutrient required for growth and development of plants. Plants take up sulfate from the soil environment through the function of plasma membrane-bound sulfate transporters expressed at the root surface cell layers. Plants then utilize the incorporated sulfate as the main sulfur source to synthesize sulfur-containing compounds such as cysteine and methionine. Measurement of root sulfate uptake capacity is essential for analyzing mutants showing altered levels of sulfate transporters and/or sulfur metabolic enzymes as a result of genetic modification or due to the effect of intrinsic or environmental factors modulating their gene expression. The method described in this protocol allows quantitative investigation of sulfate uptake rates and root-to-shoot sulfate ... [摘要]  硫是植物生长和发育所需的必需的大量营养素。植物通过在根表面细胞层处表达的质膜结合硫酸盐转运蛋白的功能从土壤环境中吸收硫酸盐。然后植物利用掺入的硫酸盐作为主要硫源合成含硫化合物,例如半胱氨酸和甲硫氨酸。测量根部硫酸盐吸收能力对于分析由于遗传修饰或由于调节它们的基因表达的内在或环境因素的影响而显示改变的硫酸盐转运蛋白和/或硫代谢酶水平的突变体是必要的。该方案中描述的方法允许使用硫酸盐作为放射性示踪剂定量研究拟南芥幼苗中的硫酸盐吸收速率和根际硫酸盐分布。该方法设计用于在幼苗阶段对多种拟南芥种子,突变体或转基因品系的平行比较。

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