| Measurement of Uptake and Root-to-Shoot Distribution of Sulfate in Arabidopsis Seedlings
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Author:
Date:
2016-01-05
[Abstract] Sulfur is an essential macronutrient required for growth and development of plants. Plants take up sulfate from the soil environment through the function of plasma membrane-bound sulfate transporters expressed at the root surface cell layers. Plants then utilize the incorporated sulfate as the main sulfur source to synthesize sulfur-containing compounds such as cysteine and methionine. Measurement of root sulfate uptake capacity is essential for analyzing mutants showing altered levels of sulfate transporters and/or sulfur metabolic enzymes as a result of genetic modification or due to the effect of intrinsic or environmental factors modulating their gene expression. The method described in this protocol allows quantitative investigation of sulfate uptake rates and root-to-shoot sulfate ...
[摘要] 硫是植物生长和发育所需的必需的大量营养素。植物通过在根表面细胞层处表达的质膜结合硫酸盐转运蛋白的功能从土壤环境中吸收硫酸盐。然后植物利用掺入的硫酸盐作为主要硫源合成含硫化合物，例如半胱氨酸和甲硫氨酸。测量根部硫酸盐吸收能力对于分析由于遗传修饰或由于调节它们的基因表达的内在或环境因素的影响而显示改变的硫酸盐转运蛋白和/或硫代谢酶水平的突变体是必要的。该方案中描述的方法允许使用硫酸盐作为放射性示踪剂定量研究拟南芥幼苗中的硫酸盐吸收速率和根际硫酸盐分布。该方法设计用于在幼苗阶段对多种拟南芥种子，突变体或转基因品系的平行比较。
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| Macromolecular Biosynthesis Assay for Evaluation of Influence of an Antimicrobial on the Synthesis of Macromolecules
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Author:
Date:
2013-06-20
[Abstract] One of the most compelling approaches in the discovery of novel antimicrobials is screening of natural sources. In our publication we report on the activity of a compound 8-hydroxyserrulat-14-en-19-oic acid (EN4), a diterpene isolated from the Australian plant Eremophila neglecta. We evaluate its applicability for treatment of implant-associated infections. A comprehensive analysis of the mechanism of action of EN4 against staphylococci revealed its membranolytic properties and a general inhibition of macromolecular biosynthesis, which was confirmed in a macromolecular biosynthesis assay and suggested a multitarget activity. The method used to investigate an influence of EN4 on the synthesis of peptidoglycan, RNA, DNA and proteins is based on precipitation of macromolecules with ...
[摘要] 在发现新的抗微生物剂中最引人注目的方法之一是筛选天然来源。在我们的出版物中，我们报告了一种化合物8-羟基胆固醇-14-烯-19-酸（EN4）（一种从澳大利亚植物中分离的二萜烯）的活性。我们评估其适用于植入物相关感染的治疗。对EN4对葡萄球菌的作用机制的综合分析揭示其膜分解性质和大分子生物合成的一般抑制，其在大分子生物合成测定中被证实并提出多靶点活性。用于研究EN4对肽聚糖，RNA，DNA和蛋白质的合成的影响的方法是基于大分子与三氯乙酸的沉淀。这些大分子由相应的[3 H] - 标记的前体合成。测量掺入和不掺入抗微生物剂的放射性，并反映测试化合物的作用模式。使用具有已知作用机制的抗生素作为对照。
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