Centrifuge 5810/ 5810 R
Company: Eppendorf
Catalog#: 5810/ 5810 R
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MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
Author:
Date:
2017-11-20
[Abstract]  Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this study, the protocol is being used to determine the cell viability of plant roots, treated with different stress inducing agents. The basis of the assay is that the dye enters the living cell’s mitochondrion where it is reduced to insoluble formazan, which ...

Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection
Author:
Date:
2017-11-20
[Abstract]  Over the past 15 years, the free-living nematode, Caenorhabditis elegans has become an important model system for exploring eukaryotic innate immunity to bacterial and fungal pathogens. More recently, infection models using either natural or non-natural nematode viruses have also been established in C. elegans. These models offer new opportunities to use the nematode to understand eukaryotic antiviral defense mechanisms. Here we report protocols for the infection of C. elegans ...

Obtaining Multi-electrode Array Recordings from Human Induced Pluripotent Stem Cell–Derived Neurons
Author:
Date:
2017-11-20
[Abstract]  Neuronal electrical properties are often aberrant in neurological disorders. Human induced pluripotent stem cells (hiPSCs)-derived neurons represent a useful platform for neurological disease modeling, drug discovery and toxicity screening in vitro. Multi-electrode array (MEA) systems offer a non-invasive and label-free platform to record neuronal evoked-responses concurrently from multiple electrodes. To better detect the neural network changes, we used the Axion Maestro MEA platform ...

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