| Differentiation of THP1 Cells into Macrophages for Transwell Co-culture Assay with Melanoma Cells
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Author:
Date:
2015-11-05
[Abstract] Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combing both targeted- and immuno-therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be differentiated into macrophages. This allows studying the effects of the macrophage secretome on cancer cells (on e.g., growth, drug response or gene expression) in co-cultures without direct cell contact interactions. This is an important aspect as it removes the presence of any phagocytic aspect to changes in the cancer cell number and behaviour. The in vitro THP-1 monocyte differentiation into polarized macrophages was used to study the effects of both M1 and M2 type populations ...
[摘要] 了解免疫细胞如巨噬细胞如何与癌细胞相互作用越来越感兴趣，因为癌症治疗趋向于在新的治疗方案中结合靶向和免疫治疗。该协议使用THP-1细胞，人类白血病单核细胞系，可以分化成巨噬细胞。这允许在没有直接细胞接触相互作用的共培养物中研究巨噬细胞分泌蛋白组织对癌细胞的作用（例如生长，药物反应或基因表达）。这是一个重要的方面，因为它消除了任何吞噬方面的癌细胞数量和行为的变化的存在。将体外THP-1单核细胞分化为极化巨噬细胞用于研究巨噬细胞的M1和M2型群体对黑素瘤细胞的作用（Smith等人，2014年， ; Tsuchiya等人，1980）。 M1型巨噬细胞通常被认为是与M2型巨噬细胞相反的肿瘤抑制，M2型巨噬细胞被认为具有组织修复和促进肿瘤生长的活性。
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| Generation and Maturation of Human Monocyte-derived DCs
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Author:
Date:
2014-08-05
[Abstract] Dendritic cells (DC) are antigen-presenting cells, which play a critical role in the regulation of the adaptive immune response. They act as a bridge between the innate and the adaptive immune systems. An approach to study their function and potentiality is to generate DC-like cells by culturing CD14+ monocyte-enriched peripheral blood mononuclear cells (PBMC). In the presence of GM-CSF and IL-4, these cultures give rise to large numbers of DC-like cells. Generating human-DC from PBMC is a useful tool to study biological functions of human DC.
[摘要] 树突状细胞（DC）是抗原呈递细胞，其在适应性免疫应答的调节中起关键作用。 它们作为先天免疫系统和适应性免疫系统之间的桥梁。 研究它们的功能和潜力的方法是通过培养富集CD14 +单核细胞的外周血单核细胞（PBMC）产生DC样细胞。 在GM-CSF和IL-4存在下，这些培养物产生大量的DC样细胞。 从PBMC产生人类DC是研究人类DC的生物学功能的有用工具。
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