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Author:
Date:
2015-11-05
[Abstract] The intestinal epithelial layer forms tubular invaginations into the underlying connective tissue of the lamina propria. These structures, termed crypts, are the basic functional unit of the intestine. Colon crypts and the surrounding lamina propria house different cell types, including epithelial cells, stem cells, enterocytes, goblet cells, as well as cells of the innate and adaptive immune systems (Clevers, 2013; Mowat and Agace, 2014). Here we describe a technique for the isolation of mouse intestinal crypt cells as well as their characterization by flow cytometry analysis (FACS) (Del Reino et al., 2012).
[摘要] 肠上皮层形成管状内陷到固有层的下面的结缔组织。 这些结构，称为隐窝，是肠的基本功能单位。 结肠隐窝和周围固有层存在不同的细胞类型，包括上皮细胞，干细胞，肠细胞，杯状细胞，以及先天和适应性免疫系统的细胞（Clevers，2013; Mowat和Agace，2014）。 在这里我们描述了用于分离小鼠肠道隐窝细胞的技术以及它们通过流式细胞术分析（FACS）的表征（Del Reino等人，2012）。
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