| Cell Wall-bound p-Coumaric and Ferulic Acid Analysis
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Author:
Date:
2016-10-05
[Abstract] Hydroxycinnamic acids, such as p-coumaric acid and ferulic acid, are a major class of compounds derived from the phenylpropanoid pathway. These compounds are widely conserved in plants and primarily accumulate in the secondary cell wall. They serve as important structural components that contribute to the overall strength and rigidity of plant cell walls and are also potent antioxidants valued for nutritional consumption. This protocol describes a method for analyzing hydroxycinnamic acids that are released after incubation under alkaline conditions.
[摘要] 羟基肉桂酸例如对 - 香豆酸和阿魏酸是衍生自苯丙素途径的一类主要化合物。 这些化合物在植物中广泛保守，并且主要累积在继发性细胞壁中。 它们作为重要的结构组分，有助于植物细胞壁的整体强度和刚性，并且是有价值的营养消耗的有效抗氧化剂。 该方案描述了用于分析在碱性条件下孵育后释放的羟基肉桂酸的方法。
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| ER Microsome Preparation and Subsequent IAA Quantification in Maize Coleoptile and Primary Root Tissue
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Author:
Date:
2016-05-05
[Abstract] Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the complexity and redundancy of the auxin biosynthetic network raises questions about control of this system. We have shown that some enzymes involved in the YUCCA-route of auxin biosynthesis are not cytosolic but localised to the endoplasmic reticulum (ER) in both Arabidopsis thaliana (YUCCA4.2) (Kriechbaumer et al., 2012) as well as Zea mays (ZmTAR1 and ZmSPI) (Kriechbaumer et al., 2015). This ...
[摘要] 生长素是植物中的主要生长激素和待发现和研究的第一种植物激素（Darwin和Darwin，1880）。植物中的植物生长素分子首先由Kögl等人确定为吲哚-3-乙酸（IAA）。 （1934）。近十年的积极研究揭示了其作用的许多分子机制，但是生长素生物合成网络的复杂性和冗余性提出了对该系统的控制的问题。我们已经显示，参与植物生长素生物合成的YUCCA途径的一些酶不是细胞质的，而是定位于拟南芥中的内质网（ER）（YUCCA4.2）（Kriechbaumer等人。（ Zm TAR1和 Zm SPI）（Kriechbaumer /em>，2015）。这提高了生长素生物合成的亚细胞区室的有趣的可能性。为了显示玉米生长素生物合成确实可以在来自玉米幼苗的微粒体以及胞质细胞部分中发生，我们应用本文所述的方案：从玉米胚芽鞘和原代根组织分离微粒体，使用色氨酸的微粒体和胞浆部分的酶测定（Trp）或吲哚-3-丙酮酸（IPyA）作为底物，并提取并定量生长素IAA。
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| Product Analysis of Starch Active Enzymes by TLC
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Author:
Date:
2015-10-20
[Abstract] Thin layer chromatography (TLC) is a useful technique for detecting the presence of monosaccharides through to oligosaccharides, though it needs to be optimized for the specific sugars that are analyzed. Here we present a method for visualizing the reaction product(s) of starch active enzymes, which can contain α-1, 4 linked and α-1, 6 linked glucose. This was first published in Molecular Microbiology (Cockburn et al., 2015). The TLC protocol is an adapted version of that published by Robyt and Mukerjea (Robyt and Mukerjea, 1994). For a summary of the products generated by starch active enzymes see the review by Hii et al. (2012).
[摘要] 薄层色谱（TLC）是用于检测单糖通过寡糖的存在的有用技术，尽管它需要针对所分析的特定糖进行优化。 在这里我们提出一种可视化的淀粉活性酶，可以包含α-1,4连接和α-1,6连接葡萄糖的反应产物的方法。 这首次发表于Molecular Microbiology（Cockburn等人，2015年）。 TLC方案是由Robyt和Mukerjea（Robyt和Mukerjea，1994）发表的适应版本。 对于由淀粉活性酶产生的产物的概述，参见Hii等人（2012）的综述。
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