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Author:
Date:
2016-06-20
[Abstract] Barley is a diploid inbreeding crop with a genome of 5 GB organized into seven chromosomes. The relatively low chromosome number and their large size make barley an excellent model for chromosome cytogenetic studies of large genome cereal crops. Chromatin can be defined as euchromatin or heterochromatin. Euchromatin is gene-rich, less condensed, and transcriptionally active while the heterochromatin is gene-poor, remains highly condensed and has low transcriptional activity (Bartova et al., 2008; Sharakhov and Sharakhova, 2015). However, the mapping of nine Histone modifications has shown that this simple description is not accurate in barley. Instead, it has been shown that combinations of histones carrying different covalent modifications reveal 10 chromatin states ...
[摘要] 大麦是一种二倍体近亲繁殖作物，基因组为5 GB，组织成7条染色体。相对低的染色体数量及其大小使大麦成为大型基因组谷物作物的染色体细胞遗传学研究的优良模型。染色质可以定义为真核细胞色素或异染色质。现代染色质是富含基因的，较少缩合的和转录活性的，而异染色质是基因缺乏的，保持高度缩合并具有低转录活性（Bartova等人。 Sharakhov和Sharakhova，2015）。然而，九个组蛋白修饰的映射显示，这种简单描述在大麦中不准确。相反，已经显示携带不同共价修饰的组蛋白的组合揭示了将大麦染色体分成三个全球环境的10个染色质状态（Baker等人，2015）。简言之，在该方案中，收集大麦根（cv Morex），在多聚甲醛中固定并压在载玻片上。使用针对特定组蛋白修饰的抗体，特别是H3K27me3，K3K27me1和H3K9me2对染色体涂片进行免疫染色。我们使用共聚焦成像获得堆叠图像，并确认这些组蛋白修饰在大麦染色体的位置。
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