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Author:
Date:
2015-09-20
[Abstract] The mitogen activated protein kinase cascade is a highly conserved signal pathway in plants. The exogenous chemicals, like hormones, can trigger a series of signalling cascades, including MAPK pathway, to modulate the plant physiology. Upon activation, some MAPKs are phosphorylated. It is important to develop methods that can detect changes in the phosphorylation status of MAPKs in plants when they come in contact with external chemicals. This method describes the exogenous treatment of Arabidopsis protoplasts with Kinetin and subsequent detection of the activated MAPKs. This method is useful for studying the effect of exogenously applied chemical compounds on the MAPK signaling cascade in Arabidopsis.
[摘要] 丝裂原活化蛋白激酶级联是植物中高度保守的信号通路。 外源化学物质，如激素，可以触发一系列信号级联，包括MAPK途径，以调节植物生理。 在激活时，一些MAPK被磷酸化。 开发能够检测植物中MAPK在与外部化学品接触时磷酸化状态的变化的方法是重要的。 该方法描述了用激动素对随后检测激活的MAPK的拟南芥原生质体的外源处理。 该方法可用于研究外源施加的化合物对拟南芥中MAPK信号级联的影响。
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Author:
Date:
2014-07-20
[Abstract] Heart failure occurring after acute myocardial infarction (MI) is among the main causes of death in western countries. Cell therapies, particularly those based on mesenchymal stem cells (MSC), represent one of the most promising approaches to repair damaged heart tissues. Several reports have provided evidences that injection of mesenchymal stem cells improved heart function following myocardial infarction (Shake et al., 2002; Zimmet and Hare, 2005; Zeng et al., 2007). Nevertheless, the mechanism(s) by which MSC exert their therapeutic action is far from being understood, and further knowledges in this field are required especially to optimize efficiency of current cardiac cell therapies. To assess the regenerative mechanisms developed by MSC in vitro, we ...
[摘要] 急性心肌梗死（MI）后发生的心力衰竭是西方国家死亡的主要原因。细胞疗法，特别是基于间充质干细胞（MSC）的那些，代表了修复受损心脏组织的最有希望的方法之一。几个报道提供了证据，注射间充质干细胞改善了心肌梗塞后的心脏功能（Shake等人，2002; Zimmet和Hare，2005; Zeng等人， 2007）。然而，MSC发挥其治疗作用的机制远未被理解，并且尤其需要在该领域中的进一步知识以优化当前心脏细胞治疗的效率。为了评估由MSC在体外形成的再生机制，我们开发了上述方法，其预期模拟梗塞心脏的典型微环境。该方法包括处于不良状态的小鼠终末分化心肌细胞与本文用作MSC模型的人多能脂肪来源干细胞（hMADS细胞）之间的物种错配共培养物。
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