| Chitin-challenged Mice Model to Study M2 Macrophages Polarization
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Author:
Date:
2015-09-05
[Abstract] Chitin is a key component of insects, fungi, and house-dust mites. Chitin has been shown to induce M2-type immune responses in vivo. Intranasal or intraperitoneal (i.p.) administration of chitin particles results in infiltration of eosinophils to the local sites and activation of macrophages with a M2 phenotype. Chitin-challenged mice model can be used to induce M2 macrophages polarization and thus to analyze the M2 phenotype from isolated peritoneal cells.
[摘要] 几丁质是昆虫，真菌和房尘螨的关键组分。 几丁质已显示在体内诱导M2型免疫应答。 鼻内或腹膜内（i.p.）施用几丁质颗粒导致嗜酸性粒细胞渗透到局部部位并激活具有M2表型的巨噬细胞。 几丁质攻击的小鼠模型可以用于诱导M2巨噬细胞极化，从而分析来自分离的腹膜细胞的M2表型。
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| Thioglycollate-elicited Peritoneal Macrophages Preparation and Arginase Activity Measurement in IL-4 Stimulated Macrophages
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Author:
Date:
2015-09-05
[Abstract] Macrophages are an essential cell population of innate immunity that plays important roles in inflammatory processes. Two main different phenotypes have been described with opposing activities: The classically activated macrophages (M1) and the alternatively activated macrophages (M2). Alternative activation of mouse macrophages can be induced by type 2 cytokines such as IL-4 and it is characterized by the regulation of the L-arginine metabolism. M2 macrophages convert arginine to ornithine and urea through the action of Arginase-1. Here we described a method for the isolation of peritoneal macrophages from thioglycollate-elicited mice and alternative activation by stimulation with IL-4. Intraperitoneal injection of thioglycollate elicits large numbers of macrophages into peritoneal ...
[摘要] 巨噬细胞是在炎症过程中起重要作用的先天免疫的必需细胞群体。 已经描述了具有相反活性的两种主要不同表型：经典激活的巨噬细胞（M1）和交替激活的巨噬细胞（M2）。 小鼠巨噬细胞的备选激活可以由2型细胞因子例如IL-4诱导，并且其特征在于L-精氨酸代谢的调节。 M2巨噬细胞通过精氨酸酶-1的作用将精氨酸转化为鸟氨酸和尿素。 在这里我们描述了从巯基乙酸诱发小鼠分离腹膜巨噬细胞和IL-4刺激的替代激活的方法。 腹膜内注射巯基乙酸盐引发大量巨噬细胞进入腹膜腔。
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