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Author:
Date:
2015-08-20
[Abstract] Tumors develop in a complex microenvironment alongside numerous cell types that impact their survival. Immune cells make up a large proportion of these accessory cells and many are known to promote tumor progression. Macrophages, in particular, are associated with poor patient prognosis and are therefore potential candidates for therapeutic targeting in cancer. However, to develop successful strategies to target macrophages, it is important to clarify whether these cells are derived from blood-borne precursors or a tissue-resident population. Parabiosis, or the surgical connection of two mice resulting in a shared blood circulation, allows the distinction between these two cellular sources. Here, we describe the use of parabiosis to define cell ontogeny in a mouse model of breast cancer.
[摘要] 肿瘤在复杂的微环境中发展，伴随许多影响其存活的细胞类型。 免疫细胞构成这些附属细胞的大部分，并且已知许多细胞促进肿瘤进展。 巨噬细胞，特别是与不良的患者预后相关，因此是癌症中治疗靶向的潜在候选者。 然而，要开发成功的策略，以靶向巨噬细胞，重要的是要澄清这些细胞是否源自血源性前体或组织驻留人群。 Parabiosis或两个小鼠的外科连接导致共享的血液循环，允许这两个细胞来源之间的区别。 在这里，我们描述在乳腺癌的小鼠模型中定义细胞个体发育的拟杆菌的使用。
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