Sodium Hypochlorite solution 10%

次氯酸钠溶液10％

Company: AppliChem

Catalog#: 211921

Similar Protocol

Simple Spectroscopic Determination of Nitrate, Nitrite, and Ammonium in Arabidopsis thaliana

Takushi Hachiya and Yuki Okamoto

Published online: 2017-05-20

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High-throughput Quantification of Ammonium Content in Arabidopsis

Author:

Izargi Vega-Mas, Asier Sarasketa and Daniel Marino,

Date:

2015-08-20

[Abstract] This protocol is a simple colorimetric assay for internal ammonium quantification in aqueous extracts from plant tissues. The method is based on the phenol hypochlorite assay (Berthelot reaction):

NH₄⁺ + hypochlorite + OH^- + phenol → indophenol

The oxidation of indophenol caused by phenol oxidation is a blue dye that is quantified at 635 nm in a spectrophotometer. Per ammonium molecule one molecule of indophenol is formed. The protocol described here is for Arabidopsis thaliana (A. thaliana) leaves and roots, although it is also valid for other plants species. [摘要] 该方案是一种简单的比色测定法，用于在植物组织的水性提取物中进行内部铵定量。该方法基于苯酚次氯酸盐测定（Berthelot反应）：
NH 4+ sup + +次氯酸盐+ OH ^- +苯酚→靛酚

由酚氧化引起的靛酚的氧化是在分光光度计中在635nm处定量的蓝色染料。每个铵分子形成一个靛酚分子。这里描述的方案是用于拟南芥（拟南芥）叶和根，虽然它也适用于其他植物物种。

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Sodium Hypochlorite solution 10%

次氯酸钠溶液10％

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