|
Author:
Date:
2015-08-05
[Abstract] XTT assay is a colorimetric method that uses the tetrazolium dye, 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide (XTT) to quantify cell-mediated damage to fungi. Actively respiring fungal cells convert the water-soluble XTT to a water-soluble, orange colored formazan product (Meshulam et al., 1995). Here, we describe the protocol that measures the ability of plasmacytoid dendritic cells (pDCs) to exert antifungal activity. This approach was first established with human polymorphonuclear cells (PMN) by Meshulam et al. (1995) and then adapted to pDC by Ramirez-Ortiz et al. (2011) and Loures et al. (2015). It can be modified for use with other effector cells and to test compounds for antifungal activity.
[摘要] XTT测定是使用四唑鎓染料，2,3-双 - （2-甲氧基-4-硝基-5-磺酰基） - （2H） - 四唑鎓-5-甲酰苯胺（XTT）定量细胞介导的损伤的比色法 到真菌。 主动呼吸真菌细胞将水溶性XTT转化为水溶性橙色甲an产物（Meshulam等人，1995）。 在这里，我们描述测量浆细胞样树突状细胞（pDCs）发挥抗真菌活性的能力的协议。 该方法首先由Meshulam等人（1995）的人多形核细胞（PMN）建立，然后由Ramirez-Ortiz等人（2011）修改为pDC， Loures 等人（2015）。 其可以被修饰以与其他效应细胞一起使用并且测试化合物的抗真菌活性。
|