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Author:
Date:
2015-07-05
[Abstract] Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality.
This protocol describes a chemical genetic screen using Arabidopsis thaliana seedlings, which has led to recognition of novel players in the plant general stress response.
[摘要] 无偏的筛选方法是能够识别生物过程中的新玩家的有力工具。 化学遗传筛选是指使用报告物应答（例如由感兴趣的启动子驱动的荧光素酶的表达）来发现当应用于植物时影响给定过程的小分子的技术。 这些化学物质因此作为用于鉴定由于基因家族冗余或突变致死性而不能通过正向遗传筛选检测的调节组分的工具。
此协议描述使用拟南芥幼苗的化学遗传屏幕，这导致在植物一般胁迫反应中的新玩家的识别。
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