| Pseudomonas syringae Flood-inoculation Method in Arabidopsis
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Author:
Date:
2017-01-20
[Abstract] Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis.
[摘要] 丁香假单胞菌 pv。 由于其在拟南芥中的致病性，已经将用于番茄细菌斑病的番茄株DC3000（ DC3000）用作模型病原体 >。 在这里，我们展示了一种基于在一半强度MS培养基上生长的幼苗拟南芥幼苗的快速可靠的接种方法。 我们还描述了一种评估拟南芥细菌生长的方法。
背景 A。 DC3000模型病理学被广泛用于研究微生物发病机制和植物先天免疫的分子机制（Ishiga等，2012和2016; Ishiga和Ichinose，2016）。 虽然已经开发了几种接种方法来研究该模型系统中的相互作用，但迄今报道的方法之一与自然界中发生的方法相似。
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| A Chemical Genetic Screening Procedure for Arabidopsis thaliana Seedlings
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Author:
Date:
2015-07-05
[Abstract] Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality.
This protocol describes a chemical genetic screen using Arabidopsis thaliana seedlings, which has led to recognition of novel players in the plant general stress response.
[摘要] 无偏的筛选方法是能够识别生物过程中的新玩家的有力工具。 化学遗传筛选是指使用报告物应答（例如由感兴趣的启动子驱动的荧光素酶的表达）来发现当应用于植物时影响给定过程的小分子的技术。 这些化学物质因此作为用于鉴定由于基因家族冗余或突变致死性而不能通过正向遗传筛选检测的调节组分的工具。
此协议描述使用拟南芥幼苗的化学遗传屏幕，这导致在植物一般胁迫反应中的新玩家的识别。
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| Nuclear Extraction from Arabidopsis thaliana
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Author:
Date:
2012-12-20
[Abstract] This protocol is to isolate nuclei from Arabidopsis cells. They can be further used for other experiments, such as nuclear protein detection, nuclear protein immunoprecipitation and so on.
[摘要] 该方案是从拟南芥细胞中分离核。 它们可以进一步用于其他实验，如核蛋白检测，核蛋白免疫沉淀等。
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