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Author:
Date:
2015-09-05
[Abstract] We recently demonstrated the presence of a quinone detoxification pathway present in Firmicutes. It is based on two enzyme activities, namely a quinone reductase, YaiB, described here, and a hydroquinone dioxygenase, YaiA, described in a separate protocol. In Lactococcus lactis (L. lactis), these enzymes are encoded by the yahCD-yaiAB operon. The operon is induced by copper to prevent the synergistic toxicity of quinones and copper. The quinone reductase, YaiB, reduces p-benzoquinone and a range of quinone derivatives to hydroquinone, using NADPH as a reductant, according to the reaction: p-benzoquinone + NADPH + H+ → hydroquinone + NADP+. We here describe the measurement of quinone reductase activity, based on the ...
[摘要] 我们最近展示了存在于Firmicutes中的醌解毒途径。 它基于两种酶活性，即本文所述的醌还原酶，YaiB和在单独方案中描述的氢醌双加氧酶YaiA。 在乳酸乳球菌（乳酸乳杆菌）中，这些酶由yahCD-yaiAB 操纵子编码。 操纵子由铜诱导以防止醌和铜的协同毒性。 使用NADPH作为还原剂，醌还原酶，YaiB，根据反应，将对苯醌和一系列醌衍生物还原为氢醌：苯醌+ NADPH + H + +氢醌+ NADP + 。 我们在这里描述醌还原酶活性的测量，基于NADPH氧化的分光光度测量。
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