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OxoidTM Agar Technical

Oxoid TM琼脂技术

Company: Thermo Fisher Scientific
Catalog#: LP0013
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Virus-induced Gene Silencing (VIGS) in Barley Seedling Leaves
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Date:
2015-06-20
[Abstract]  Virus induced gene silencing (VIGS) is one of the most potent reverse genetics technologies for gene functional characterisation. This method exploits a dsRNA-mediated antiviral defence mechanism in plants. Using this method allows researchers to generate rapid phenotypic data in a relatively rapid time frame as compared to the generation of stable transformants. Here we describe a simple method for silencing a target gene in barley seedling leaves using vectors based on the Barley Stripe Mosaic Virus (BSMV). [摘要]  病毒诱导的基因沉默(VIGS)是基因功能表征的最有效的反向遗传学技术之一。 这种方法利用dsRNA介导的抗病毒防御机制在植物中。 与稳定转化体的产生相比,使用该方法允许研究人员在相对快的时间框架中产生快速表型数据。 在这里我们介绍了一种简单的方法,使用基于大麦条纹花叶病毒(BSMV)的载体,在大麦幼苗叶片中沉默目标基因。

Visual Assessment of the Severity of Fusarium Seedling Blight (FSB) and Fusarium Head Blight (FHB) Disease in Barley
Author:
Date:
2015-06-20
[Abstract]  Fusarium pathogens are among the most damaging pathogens of cereals. These pathogens have the ability to attack the roots, seedlings, and flowering heads of barley and wheat plants (Simpson et al., 2004). Resulting in yield loss and head blight disease and also resulting in the contamination of grain with mycotoxins harmful to human and animal health (McMulen et al., 1997; Walter et al., 2010; Agostinelli et al., 2012). The study of Fusarium diseases, including host disease resistance and the effect of exogenous agents (chemicals, biocontrol agents, etc.), requires robust and effective methods for the assessment and quantification of visual disease symptoms. Here we describe the methods commonly used for the assessment and ... [摘要]  镰孢属病原体是谷类中最具破坏性的病原体之一。 这些病原体具有攻击大麦和小麦植物的根,幼苗和开花头的能力(Simpson等人,2004)。 导致产量损失和赤霉病,并且还导致谷粒被对人类和动物健康有害的霉菌毒素的污染(McMulen等人,1997; Walter等人, ,2010; Agostinelli et al。,2012)。 镰刀菌属疾病的研究,包括宿主疾病抗性和外源性试剂(化学品,生物控制剂等)的影响,需要用于评估和定量视觉疾病症状的强大且有效的方法。 在这里我们描述了通常用于评估和量化镰刀菌枯萎病和镰孢属赤霉病的严重性的方法。

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