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RPMI 1640 Medium

RPMI 1640 Medium

Company: Thermo Fisher Scientific
Catalog#: 11875085
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Hanging Drop Aggregation Assay of Breast Cancer Cells
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2015-02-05
[Abstract]  Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method requires no specialized equipment and provides a means of generating tissue-like cellular aggregates for measurement of biomechanical properties for molecular and biochemical analysis in a physiologically relevant model. [摘要]  悬滴测定可用于通过在生理条件下产生3D球体来研究细胞 - 细胞内聚性和细胞 - 基质粘附。 它还可以用于阐明细胞 - 细胞或细胞-ECM相互作用在指定两种(或更多种)不同细胞群体之间的空间关系中的作用。 这种简单的方法不需要专门的设备,并且提供了在生理相关模型中产生用于测量生物力学性质的组织样细胞聚集体用于分子和生物化学分析的方法。

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