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Author:
Date:
2015-05-05
[Abstract] We have developed an unsophisticated, non-disruptive and accurate method for evaluation of pathogen colonization in planta. In this protocol we use a Ralstonia solanacearum (R. solanacearum) UY031 strain genetically modified to constitutively generate light from a synthetic luxCDABE operon stably inserted in its chromosome. This system allows bacterial quantification in a high-throughput manner, avoiding time-consuming and tedious bacterial dilution plating and colony counting. In addition, this system could be especially useful in plant breeding programs to detect bacterial latent growth in symptomless parental lines before their inclusion in long-term disease resistance breeding programs.
[摘要] 我们已经开发了一种不成熟的，非破坏性和准确的方法用于评估植物中的病原体定居。 在该方案中，我们使用经基因修饰以从稳定插入的合成的luxCDABE 操纵子组成性地产生光的（R）solanacearum 其染色体。 该系统允许以高通量方式进行细菌定量，避免耗时和繁琐的细菌稀释电镀和菌落计数。 此外，该系统可以在植物育种程序中特别有用，以在其包括在长期疾病抗性育种程序中之前检测无症状亲本细胞中的细菌潜伏生长。
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