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Brassinazole

Brassinazole

Company: TCI America
Catalog#: B2829
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Protocol to Treat Seedlings with Brassinazole and Measure Hypocotyl Length in Arabidopsis thaliana
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Date:
2015-08-20
[Abstract]  The plant hormones brassinosteroids (BR) promote hypocotyl elongation of Arabidopsis thaliana (A. thaliana) seedlings both under light and dark (etiolated) conditions. A common assay to determine if a mutant or transgenic line is affected in BR biosynthesis or response is a sensitivity assay to brassinazole (BRZ), an inhibitor of P450 cytochromes specific to BR biosynthesis.

Here we provide a protocol to compare BRZ sensitivity of different A. thaliana genotypes in terms of hypocotyl elongation (Bernardo-García et al., 2014).
[摘要]  植物激素油菜素类固醇(BR)在光和黑暗(etiolated)条件下促进拟南芥(
a thaliana )幼苗的下胚轴延伸。 确定突变体或转基因品系是否受BR生物合成或反应影响的常用测定法是对BR生物合成特异性的P450细胞色素抑制剂 - 芸苔素(BRZ)的敏感性测定。
在这里,我们提供一个协议来比较不同 A的BRZ灵敏度。 (Bernardo-García等人,2014)。

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