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1-Naphthyl palmitate

棕榈酸1-萘酯

Company: Sigma-Aldrich
Catalog#: N9875
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In Gel Detection of Lipase Activity in Crude Plant Extracts (Olea europaea)
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Date:
2015-04-20
[Abstract]  Here, we provide a detailed protocol describing a SDS-PAGE based procedure to assay in gel neutral lipase activity. Total protein extracts are separated by SDS-PAGE and gels are treated with lipase substrate-α-naphthyl palmitate. This long-chain fatty acid ester is hydrolysed by lipases present in the gel. The product resulting from this reaction can be then visualized in the gel as yellow-brownish activity bands. This relatively simple and effective method of lipase assay detection can be used for crude protein extracts from different plant tissues. [摘要]  在这里,我们提供详细的协议描述基于SDS-PAGE的程序来测定凝胶中性脂肪酶活性。 通过SDS-PAGE分离总蛋白提取物,并用脂肪酶底物-α-棕榈酸萘酯处理凝胶。 这种长链脂肪酸酯被存在于凝胶中的脂肪酶水解。 由该反应产生的产物然后可以在凝胶中显现为黄棕色活性带。 这种相对简单和有效的脂肪酶测定法可用于来自不同植物组织的粗蛋白提取物。

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