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Blue screw caps

蓝色螺帽

Company: Agilent Technologies
Catalog#: 5185-5820
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A Gas Chromatography-Mass Spectrometry-Based Two Stage Assay for Measurement of in vitro myo-Inositol 3-phosphate Synthase (INO1) Activity
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2015-03-05
[Abstract]  This method describes an in vitro assay for measuring INO1 enzyme activity (the conversion of glucose 6-phosphate to myo-inositol 3-phosphate) in cell-free extracts. The method was first described for Plasmodium falciparum cells in MacRae et al. (2014) and consists of two parts: Part 1 describes the assay itself while part 2 describes analysis of the myo-inositol 3-phosphate product using gas chromatography-mass spectrometry (GC-MS). [摘要]  该方法描述了用于测量无细胞提取物中INO1酶活性(葡萄糖-6-磷酸转化为肌醇-3-磷酸)的体外测定。 该方法首先在MacRae等人(2014)中描述了恶性疟原虫细胞,并由两部分组成:第1部分描述测定本身,而第2部分描述分析 使用气相色谱 - 质谱(GC-MS)测定肌醇3-磷酸产物。

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