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BD Precisionglide® syringe needles

注射器针

Company: Sigma-Aldrich
Catalog#: Z192392
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Macrophage Phagocytosis Assay of Staphylococcus aureus by Flow Cytometry
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Date:
2015-02-20
[Abstract]  This protocol describes a straightforward technique to evaluate the phagocytotic capacity of murine macrophages for Staphylococcus aureus (S. aureus). By staining S. aureus with Hexidium Iodide and staining murine bone marrow-derived macrophages (BMDMs) with FITC, the macrophage bacterial up-taking ability can be rapidly analyzed by flow cytometry. S. aureus is a Gram-positive bacteria causing severe human and animal infections. Host immune cells such as macrophages serve to eliminate S. aureus by phagocytosing the pathogen and save the host from life-threatening diseases. Study of host macrophage ability to phagocytose S. aureus is important for understanding the host-pathogen interaction and can help to elucidate the pathogenesis of S. ... [摘要]  该协议描述了评估小鼠巨噬细胞对金黄色葡萄球菌(金黄色葡萄球菌)的吞噬能力的直接技术。 通过染色。 金黄色葡萄球菌与碘化己锭染色并用FITC染色鼠骨髓衍生的巨噬细胞(BMDM),可以通过流式细胞术快速分析巨噬细胞细菌摄取能力。 aureus 是一种革兰氏阳性菌,引起严重的人类和动物感染。 宿主免疫细胞例如巨噬细胞用于消除S。 金黄色葡萄球菌通过吞噬病原体并将宿主从危及生命的疾病中拯救。 宿主巨噬细胞吞噬能力的研究。 金黄色葡萄球菌对于理解宿主 - 病原体相互作用是重要的,并且可以帮助阐明S的发病机制。 aureus 感染。 该方案也可应用于其他革兰氏阳性菌的巨噬细胞吞噬测定。

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