| RNA Strand Displacement Assay for Hepatitis E Virus Helicase
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Author:
Date:
2017-04-05
[Abstract] The hepatitis E virus (HEV) helicase uses ATP to unwind the RNA duplexes. This is an essential step for viral replication. This protocol aims to measure the double strand RNA unwinding activity of the HEV helicase.
[摘要] 乙型肝炎病毒（HEV）解旋酶使用ATP来解开RNA双链体。 这是病毒复制的重要步骤。 该方案旨在测量HEV解旋酶的双链RNA展开活性。
已经使用放射性标记的双链RNA（dsRNA，Karpe等人，2010）测量了HEV解旋酶的RNA展开活性。 我们已经建立了用于测量HEV解旋酶的dsRNA展开活性的非放射性测定方案。 该测定利用荧光标记的RNA来测量从人肝癌细胞纯化的HEV解旋酶蛋白的活性，从而消除了处理放射性物质的需要。
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| Experimental Pipeline for SNP and SSR Discovery and Genotyping Analysis of Mango (Mangifera indica L.)
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Author:
Date:
2016-08-20
[Abstract] Establishing a reservoir of polymorphic markers is an important key for marker-assisted breeding. Many crops are still lack of such genomic infrastructure. Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) are useful as markers because they are widespread over the genome and many technologies were developed for high throughput genotyping. We present here a pipeline for developing a reservoir of SNP and SSR markers for Mangifera indica L. as an example for fruit tree crops having no genomic information available. Our pipeline includes de novo assembly of reference transcriptome with MIRA and CAP3 based on reads produced by 454-GS FLX technology; Polymorphic loci discovery by alignment of Illumina resequencing to the transcriptome reference; ...
[摘要] 建立多态性标记的储库是标记辅助育种的重要关键。许多作物仍然缺乏这样的基因组学基础设施。单核苷酸多态性（SNP）和简单序列重复（SSR）可用作标记，因为它们在基因组上广泛存在，并且开发了许多技术用于高通量基因分型。我们在这里提出了用于开发Mangifera indica的SNP和SSR标记库的管道，作为没有基因组信息的果树作物的实例。我们的管道包括基于由454-GS FLX技术产生的读数的具有MIRA和CAP3的参考转录组的 de novo 装配;通过Illumina重排序到转录组参考的比对的多态性基因座发现;通过用Fluidigm技术进行基因分型，鉴定整个种质资源集合中多态的基因座子集。 [背景] ...
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| Identification of Natural Hybrids by SSR Markers in Mussaenda
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Author:
Date:
2016-07-05
[Abstract] Detection of natural hybrids is of great significance for plant taxonomy, reproductive biology, and population genetic studies. Compared with methods depending on morphological characters, molecular markers provide reliable and much more accurate results. This protocol describes approaches employing microsatellite (SSR) markers to identify inter-specific hybrids in Mussaenda (Rubiaceae).
[摘要] 自然杂交的检测对植物分类学，生殖生物学和群体遗传学研究具有重要意义。 与依赖于形态特征的方法相比，分子标记提供可靠和更准确的结果。 该协议描述了采用微卫星（SSR）标记来鉴定Mussaenda（茜草科）中的特异性杂交体的方法。
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