Calcium chloride solution

氯化钙溶液

Company: Sigma-Aldrich

Catalog#: 21114

Similar Protocol

Isolation and Enrichment of Major Primary Neuroglial Cells from Neonatal Mouse Brain

Santosh Kumar Samal, Madhav Sharma and Jayasri Das Sarma

Published online: 2024-01-20

Bio-protocol()

Company-protocol()

Other protocol()

Organotypic Spinal Cord Slice Cultures and a Method to Detect Cell Proliferation in These Slices

Author:

Jillian M. Daniel, Jim Deuchars and Susan A. Deuchars,

Date:

2016-10-05

[Abstract] In these culture models, the normal cytoarchitecture and local neuronal circuits of the spinal cord are preserved, offering a compromise between dissociated cell cultures and complete animal studies. The addition of 5-ethynyl-2’-deoxyuridine (EdU) to the culture medium allows for the detection of proliferating cells. [摘要] 在这些文化模型中，保留了脊髓的正常细胞结构和局部神经元回路，提供了解离的细胞培养物和完全动物研究之间的折衷。向培养基中加入5-乙炔基-2'-脱氧尿苷（EdU）允许检测增殖细胞。

RNA Chromatin Immunoprecipitation (RNA-ChIP) in Caenorhabditis elegans

Author:

Germano Cecere and Alla Grishok,

Date:

2014-12-20

[Abstract] The RNA chromatin immunoprecipitation assay (RNA-ChIP) allows detection and quantification of RNA–protein interactions using in vivo cross-linking with formaldehyde followed by immunoprecipitation of the RNA–protein complexes. Here we describe the RNA–ChIP protocol that we have adapted for Caenorhabditis elegans (C. elegans) to detect interaction between the nuclear Argonaute CSR-1 (chromosome segregation and RNAi deficient) protein and its target nascent RNAs. We have used a transgenic strain expressing a recombinant long isoform of CSR-1 protein fused with N-terminal 3x FLAG epitope. [摘要] RNA染色质免疫沉淀测定（RNA-ChIP）允许使用体内与甲醛交联，随后RNA-蛋白复合物的免疫沉淀来检测和定量RNA-蛋白质相互作用。在这里我们描述我们已经适应于秀丽隐杆线虫（ C. elegans ）的RNA-ChIP协议以检测核Argonaute CSR-1（染色体分离和RNAi缺陷）蛋白及其目标新生RNA。我们使用表达与N-末端3x FLAG表位融合的CSR-1蛋白的重组长同种型的转基因菌株。

Comments

Calcium chloride solution

氯化钙溶液

免责声明