| In vitro CLE Peptide Bioactivity Assay on Plant Roots
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Author:
Date:
2015-12-20
[Abstract] Plant CLAVATA3/ESR (CLE)-related proteins play diverse roles in plant growth and development including regulating the development of root meristem. The mature functional forms of CLE peptides are typically 12-13 amino acids (aa) in length that are derived from the conserved C-termini of their precursor proteins. Genes encoding small secreted peptides sharing similarity to plant CLE proteins have recently been cloned from plant-parasitic nematodes, pests that infect many important crops. It is demonstrated that exogenous application of synthetic 12-14 aa CLE peptides corresponding to the CLE domain of their precursor proteins can suppress plant root growth. This protocol is to evaluate the bioactivity of CLE peptides originated from plant-parasitic nematodes by measuring the growth of ...
[摘要] 植物CLAVATA3/ESR（CLE）相关蛋白质在植物生长和发育中发挥多种作用，包括调节根分生组织的发育。 CLE肽的成熟功能形式通常为12-13个氨基酸（aa）长度，其衍生自其前体蛋白的保守C-末端。 最近从植物寄生线虫，感染许多重要作物的害虫克隆了编码与植物CLE蛋白相似的小分泌肽的基因。 证明外源应用对应于其前体蛋白的CLE结构域的合成12-14aa CLE肽可以抑制植物根生长。 该协议是通过测量CLE肽处理后植物根的生长或根尖分生组织（RAM）的大小来评价源自植物寄生线虫的CLE肽的生物活性。 用于研究的植物包括拟南芥和马铃薯。
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| Purification of Tumor-Associated Macrophages (TAM) and Tumor-Associated Dendritic Cells (TADC)
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Author:
Date:
2014-11-20
[Abstract] Tumors are heterogeneous microenvironments where complex interactions take place between neoplastic cells and infiltrating inflammatory cells, such as tumor-associated macrophages (TAM) and tumor-associated dendritic cells (TADC). The relevance of tumor-infiltrating mononuclear myeloid cells is underscored by clinical studies showing a correlation between their abundance and poor prognosis (Laoui et al., 2011). These cells are able to promote tumor progression via several mechanisms, including induction of angiogenesis, remodeling of the extracellular matrix, stimulation of cancer cell proliferation and metastasis and the inhibition of adaptive immunity (Laoui et al., 2011). Moreover, mononuclear myeloid cells are characterized by plasticity and versatility in response ...
[摘要] 肿瘤是异质微环境，其中在肿瘤细胞和浸润性炎症细胞例如肿瘤相关巨噬细胞（TAM）和肿瘤相关树突细胞（TADC）之间发生复杂的相互作用。临床研究强调了肿瘤浸润性单核骨髓细胞的相关性，显示其丰度与不良预后之间的相关性（Laou等人，2011）。这些细胞能够通过几种机制促进肿瘤进展，包括诱导血管生成，重塑细胞外基质，刺激癌细胞增殖和转移以及抑制适应性免疫（Laoui等人，2011 ）。此外，单核骨髓细胞的特征在于响应于微环境信号的可塑性和通用性，导致不同的活化状态，如通过在肿瘤中存在不同的功能性TAM亚型所说明的（Movahedi等人，2010; Laoui et al。，2014）。在这里，我们描述了允许其分子和功能表征的TAM和TADC的有价值的分离技术。
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| In vitro Nematode Infection on Potato Plant
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Author:
Date:
2014-01-05
[Abstract] Potato cyst nematodes (PCNs; Globodera rostochiensis and G. pallida) are devastating pests that infect potato root. We describe an in vitro assay for PCN infection on potato plantlet in tissue culture. This method is useful for studying nematode parasitism on potato and for investigating responses of potato clones/lines to PCN infection.
[摘要] 马铃薯胞囊线虫（PCNs; Globodera rostochiensis 和 G。pallida ）是感染土豆根的毁灭性害虫。 我们描述了在组织培养的马铃薯小植株上的PCN感染的体外测定。 这种方法可用于研究马铃薯上的线虫寄生和调查马铃薯克隆/株系对PCN感染的反应。
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